Figure 3. Survival signaling pathways in neuroretina from diabetic patients. A: Protein extracts were prepared from neuroretina from diabetic patients (n=5) and nondiabetic control individuals (n=5).
Total protein (50 μg) was used for western blot analysis with the antibodies against B-cell lymphoma-extra large (BclxL),
FLICE-like inhibitory protein (FLIP) and ciclooxygenase-2 (COX2). Anti-GADPH antibody was used as a loading control. B: Autoradiograms were quantified with scanning densitometry. Results are expressed as arbitrary units of protein expression
and are means±SD. C: nondiabetic donors, D: diabetic donors.