Figure 2. Viability of cultured ocular cells exposed to 0.5 µg/ml verteporfin with different intensities of laser light. The percent of live cells was determined with 3-(4,5- dimethyl-2-thiazoyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay in human scleral fibroblasts (hFibro), pig trabecular meshwork cells (pTMC), human trabecular meshwork cells (hTMC), and a human retinal pigment epithelial cell line (ARPE-19). Data were normalized to cells not treated with either verteporfin or laser light (0 µg/ml + 0 µJ/cm²; 100% Live), and plotted as the mean (n=4) with error bars representing the standard deviation. *=p<0.05 versus both 0 µg/ml + 0 µJ/cm² and pretreat + 50 µJ/cm².