Figure 1. Expression of and changes in the CB₁ receptor in human retinal pigment epithelial (RPE) cells. A: In the western blot analysis of CB₁ receptor protein expression changes in primary RPE cells, CB₁ receptor protein level was significantly increased by H₂O₂ incubation in a dose-dependent manner. B: CB₁ receptor protein localized to the cytoplasm and cellular membrane as demonstrated with immunofluorescence staining (Bar=20 μm). Quantitative analysis of the fluorescent levels is indicated in the right panel. *p<0.05 versus control, the sample number is n=5 per group and we performed t test here. C: A representative photograph of primary cultured RPE cells seeded for 24 h.