E-cadherin particle internalization from the cell border to the interior in HCLE was significantly reduced in contrast to the increased internalization of ShCdk5 and olomucine (*p≤0.05; a). The length of fast moving particles analyzed within the stipulated time for all the particles was 18um (b). Among the fast moving particles, a subset of particles ≥20um length internalized was significantly increased only in the ShCdk5 and olomucine (c). E-cadherin particle movement analyzed using TIRF therefore suggests that the particle rate of internalization and proportion of particles being internalized increased in the absence of Cdk5 Derivation: a. Average straight distance=Sum of straight distances/total number of straight distance. Internalized E- cadherin=particle movement b. Average Fast Moving Particles (distance ≥3μm,tortuosity ≤1.5)=sum of all the fast moving particles/tolat no. of fast moving particles. c. Proportion of fast moving internalized particles=No. of Fast moving Particles/total no of particles analyzedx100 (i) % Subset of fast moving internalized particles=No of fast moving particles at straight distance≥20µm tortuosity ≤1.5/ total no of fast moving particles x100 (ii). Results are expressed as average ± standard error mean.