Figure 7. Rho activity mediates the downstream regulation of CDK5 in promoting cell–cell junction formation. Cell aggregates were measured with and without Ca²⁺ and defined as particle size in μm² using NIH image J. In the presence of the C3 transferase, a Rho kinase inhibitor, the human corneal limbal epithelial (HCLE) cells form larger cell aggregates (A), and there is a reduction in the particle size in the presence of CDK5 and Rho kinase inhibitors suggesting the role of Cdk5 downstream signaling via the Rho kinase in the cell–cell junction formation (C). Olomoucine alone (E) reduces the formation of cell–cell aggregates than the control untreated (G) HCLE. Ratio of particle size=particle size (um) in the presence of calcium/ particle size (um) without calcium is represented in the graph (I). A–H are 20X images.