Figure 3. Cell–cell adhesions in the ShCDK5 cells. Confluent human corneal limbal epithelial (HCLE) and ShCDK5 cells immunostained with E-cadherin. The HCLE cells show border localization of E-cadherin (A). ShCDK5 increases the border localization to the cell interior marked by punctuate staining (arrows) within the cells. The ratio of the border to cell interior was significantly reduced in the ShCDK5 E-cadherin-labeled cells (0.347±0.145) when compared to the HCLE (0.535±0.142) 100X images.