Figure 2. Induction of pentraxin 3 (PTX3) protein expression by interleukin 1 beta (IL-1β) and tumor necrosis factor alpha (TNF-α) in human RPE cells. The expression of PTX3 protein levels was assayed in the absence or presence of IL-1β (A) and TNF-α (B) in ARPE-19 cells. After 24 h treatment of IL-1β or TNF-α, PTX3 protein levels were analyzed with western blotting analysis with a human anti-PTX3 polyclonal antibody. These experiments were performed at three independent times, and the fold change in protein levels was quantitated as signal intensity corrected for loading in vehicle cells (white bars) or cells exposed to IL-1β (black bars) or TNF-α (black bars). Values are mean±SD. Results are representative for three independent experiments. *p<0.05 versus vehicle.