Molecular Vision: Chen, Mol Vis 2013; 19:292-302. Table 1

Table 1. Primers used to amplify the genomic fragments of OPA1.

Fragment	Forward primer (5′-3′)	Reverse primer (5′-3′)	Product length (bp)	Annealing temperature
Exon 1	CCTCGGCCGCGGCTCTGTGC	GGGCTCCTGTCATTCTGGGTCCTCAAG	327	65 °C
Exon 2	TTTGCACCACATTTTCCTCATCT	GGCATCTTCCTATTAGCATCATTA	743	60 °C
Exon 3	GGGCAAAATTATGAAACCT	TAAAATTATGGGCTACTGG	523	57 °C
Exon 4	ACTGCCTGGGCTGGAAC	GGAACTGTCATTTTACTGGGC	507	65 °C
Exon 4b	GCCCTATCGTAATATGAAATCTGAG	GCATAAGCAGCATTATAAATTTGG	257	60 °C
Exon 5	AGGCGATTTGATTCTTTGA	ACTTGGATGTTTTTGTATTTG	320	55 °C
Exon 5b	AACCATCCCTCCCTAGCTTACATCT	GGCTTTACCTATACTACCCACTCCAG	396	65 °C
Exon 6	CTTTCATAAGAAATGACTAGAATAGCAACA	TGGGCATAAGATTCACTCAAAATAGG	560	57 °C
Exon 7	ATGTGAGTAGCAAGGAATTTTCCAAGTG	CCTCCAAGCACATTAGGTTAGAAAGAAA	484	65 °C
Exon 8	CTAAATAAACTGAATGAGAAATGGAC	ACATTACTTGGAACATGTAAGATTAC	446	60 °C
Exon 9	GTTTTGCTGTTCCTATTTTCAATGTGCA	GCCTCCTGGCTGTGCCTTCTACTGATTT	464	65 °C
Exon 10–11	CCCAGCAGTAGTGTGAAGGG	AAAACAATGCTAAAGTTTGGGG	716	60 °C
Exon 12–13	TGTGAGCGTCTTATCTGAATGGA	AATGAATACGAAGAGAAGGCAAAAA	506	60 °C
Exon 14	TTGCTATAATGTAGACACAGGGG	CCATGTACCATTTCCTTTTGTG	395	60 °C
Exon 15–16	TCATTCCGGGTTTTCGATAC	AAACTGCTCTCAATTCTGCC	662	65 °C
Exon 17	GCTACCGTATTGGAATGTTTTCCTCCTC	AAATGTTCTCATCTGTTTGAACTCTGCA	525	65 °C
Exon 18	GGGTCATAGGCGCACTCTC	TCTCAGAAAACACTTTCAACACTTG	425	57 °C
Exon 19	CCCAAATTCAGCCTAGTCAAAAA	GAGCCAAGGCAACAATAAATCAC	293	65 °C
Exon 20	GCTGGAGTGGAAGAACAAAGACAAA	CCCAAAACAGAGATGAGGAATAAAGAA	616	65 °C
Exon 21	CCATATCTGTCCCCAGCAAC	GCAACAGGTGATTTTAGAAGGG	541	65 °C
Exon 22	TAACAAATAAGCAGGCAAGTAAAAGAAG	CATTGGTTTTAGTAGTTACAAAGCAGTT	464	60 °C
Exon 23	ATGTGGGTTTTTTCCTTTA	GCATGTTTCATCTCTTGTC	448	57 °C
Exon 24	TGATTAAGCTTGTGTTATCTTTTATGC	CGTGACAAAAGTCAAATTAAGCAC	372	60 °C
Exon 25	CCTACCCTGTCTACTCCACAAG	TTTCCCCAGATGATCAAAGG	523	60 °C
Exon 26	TTAAGCTTAGGACATATCTACTGGTTC	TGGGAAGTATTTTGGCATCC	291	60 °C
Exon 27	TTTTGGGAAATCTGCACTTC	TCTGACCTTGTTTTCCACCC	533	60 °C
Exon 28	TTGGGTAAAAGGTGGTATGGTGAG	CAAGCAGGATGTAAATGAAGCAGAA	309	65 °C