Table 1 of Giblin, Mol Vis 2013; 19:267-280.

Table 1. Numbers of total crystallin spectral counts (an indication of relative crystallin abundance), cysteine-containing peptide spectral counts, number of –SH groups for each crystallin, and numbers of –SH groups bound by GSH or cysteine for each guinea pig lens water-soluble nuclear crystallin.

Crystallin* Total spectral counts** peptide counts as % of total Cysteine-containing peptide counts** Cysteine-peptide counts as % of total Number of -SH groups per crystallin -SH groups bound by GSH or cysteine***
ζ 4500 15.9 828 10.4 5 4
γS 2947 10.4 695 8.7 7 5
βB2 2794 9.9 516 6.4 2 2
αA 2722 9.7 510 6.4 1 1
γB 2664 9.4 1240 15.5 7 5
βB1 2299 8.2 865 10.8 5 4
γC 2226 7.9 680 8.5 7 5
βA4 1874 6.6 479 6 4 4
βA1/A3 1757 6.2 1173 14.7 8 6
αB 1597 5.7 0 0 0 0
βB3 1306 4.6 159 2 3 2
γA 922 3.3 709 8.9 7 4
βA2 613 2.2 140 1.7 5 2
total 28221 100 7994 100 61 44

* γN-crystallin was not included since it had very low numbers (<50) of MS/MS spectral counts. **Averages of control and hyperbaric oxygen-treated results. *** The number of –SH groups in either control or hyperbaric oxygen-treated samples that were found to bind either glutathione or cysteine.

Giblin, Mol Vis 2013; 19:267-280. http://www.molvis.org/molvis/v19/267

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