Figure 4 of Giblin, Mol Vis 2013; 19:267-280.


Figure 4. The numbers of tandem mass (MS/MS) spectra are shown for the 5 -SH group-containing peptides of guinea pig lens water-soluble nuclear βB1-crystallin. A shows total counts for each of the 5 peptides containing an -SH group; B shows peptides containing protein-bound glutathione (PSSG) expressed as per -SH peptide; and C shows peptides containing protein-bound cysteine (PSSC) expressed as per -SH peptide. Open bars are counts from age-matched controls and solid black bars are counts after 30 treatments of the animals with hyperbaric oxygen. There is a different vertical scale for A, compared to those for B and C, which are identical. The counts in A correspond to a soluble protein sample of 0.4 mg. Peptides for each of the 5 cysteine residues showed an O2-induced decrease in number of counts (A). The majority of O2-induced PSSG (B) and PSSC (C) were shown by residues C148 and C176.