Figure 8. Representation of a 6.5 kb insertion in intron 1 of Gpr179 identified with polymerase chain reaction using an intron-specific primer and subsequent Sanger sequencing. The schematic indicates the presence of the mutation in intron 1 of Gpr179. The sequence obtained with PCR with an intron-specific primer (Gpr179 rev) was used to design the mutation-specific primer. All three Gpr179 primers (Table 1) were used for genotyping.