Representation of a 6.5 kb insertion in intron 1 of Gpr179
identified with polymerase chain reaction using an intron-specific primer and subsequent Sanger sequencing. The schematic
indicates the presence of the mutation in intron 1 of Gpr179
. The sequence obtained with PCR with an intron-specific primer (Gpr179
rev) was used to design the mutation-specific primer. All three Gpr179
primers (Table 1
) were used for genotyping.