Figure 6. Calponin and F-actin immunolocalization in the trabecular meshwork (TM) and ciliary muscle (CM). A: Positive calponin labeling was seen in the CM posteriorly (opened arrows). B: Positive F-actin labeling (red) was seen in ciliary processes, CM and TM. C: Calponin co-localized with F-actin in the CM. D: Bright field for tissue orientation. E: merged bright field and fluorescence images provide detailed structural orientation. F: Magnified image of dotted square of C showed only spotty calponin co-localization with F-actin in TM cellular layers next to SC (dotted outline, asterisk*). Scale bars, 20 μm.