Figure 5. Non-muscle myosin heavy chain (MHC) and F-actin immunolocalization in trabecular meshwork (TM) and ciliary muscle (CM). A: Positive MHC labeling was primarily in the TM (closed arrows). MHC labeling was of relatively low intensity or absent in the CM (opened arrows), especially posteriorly. B: Positive F-actin labeling (red) was seen in ciliary processes, CM and TM. C: Patchy co-localization of MHC and F-actin was seen in TM cellular layers. D: Bright field for tissue orientation. E: Merged bright field and fluorescence images provide detailed structural orientation. F: Magnified image of dotted square of C showed co-localization of MHC and F-actin in CM. In the TM, adjacent to SC (dotted outline with asterisk*), MHC and F-actin co-localization was spotty. Scale bars, 20 μm.