Figure 1. Results of nitroblue tetrazolium (NBT) salt reduction assay in the ocular lens of Wistar rat pups. A: No blue color formation was observed upon incubation with NBT for 45 min in Group I rat lenses. B: Formation of intensely blue-colored formazan deposits, suggestive of superoxide (O2 −) generation was observed in Group II rat lenses. C: Negligible blue formazan deposition was observed in Group III rat lenses.