Figure 2. D6^−/− dendritic cells generate impaired alloreactive T-cell responses. A: Mixed leukocyte reaction (MLR) assay of bone marrow (BM)-derived mitomycin C–treated dendritic cells (DCs) from wild-type (WT; D6^+/+) or D6^−/− mice. Cells were cocultured with allodisparate T cells (BALB/c), and less T-cell proliferation was evaluated using the bromodeoxyuridine (BrdU) assay. B: Delayed-type hypersensitivity (DTH) reaction in WT BALB/c mice receiving either splenic T cells from BALB/c mice (1×10⁵) in 10 μl Hanks’ balanced salt solution alone or with 1×10⁵ BM-derived DCs of either D6^−/− or WT C57BL/6 mice. Cells were injected into the right pinna, and ear thickness was measured 48 h after injection. The experiment was performed twice, and representative data of one experiment are shown here; mean±standard error of the mean (SEM) of n=5 mice per group. P values are indicated comparing WT with D6^−/− mice: **p≤0.01.