Figure 2. D6−/− dendritic cells generate impaired alloreactive T-cell responses. A: Mixed leukocyte reaction (MLR) assay of bone marrow (BM)-derived mitomycin C–treated dendritic cells (DCs) from wild-type
(WT; D6+/+) or D6−/− mice. Cells were cocultured with allodisparate T cells (BALB/c), and less T-cell proliferation was evaluated using the bromodeoxyuridine
(BrdU) assay. B: Delayed-type hypersensitivity (DTH) reaction in WT BALB/c mice receiving either splenic T cells from BALB/c mice (1×105) in 10 μl Hanks’ balanced salt solution alone or with 1×105 BM-derived DCs of either D6−/− or WT C57BL/6 mice. Cells were injected into the right pinna, and ear thickness was measured 48 h after injection. The experiment
was performed twice, and representative data of one experiment are shown here; mean±standard error of the mean (SEM) of n=5
mice per group. P values are indicated comparing WT with D6−/− mice: **p≤0.01.