Figure 3. The effects of chrysene (Chry) on lactate dehydrogenase (LDH) levels in MIO-M1 cells. The MIO-M1 cells treated 24 h with 1,000 μM chrysene showed increased LDH levels activity (***p<0.001) compared to cells treated with the dimethyl sulfoxide (DMSO)-equivalent. The untreated cells, 500 μM-chrysene-treated cells, 300-μM-chrysene-treated cells, 100-μM-chrysene-treated cells, and their DMSO- equivalent-treated cells showed low levels of LDH. Assays were performed in triplicate and repeated three times. Assays were performed in triplicate and the experiments repeated three times. Values are mean ± standard error mean (SEM).