Figure 1 of Mansoor, Mol Vis 2013; 19:25-38.

Figure 1. The effects of chrysene on MIO-M1 cell viability. A: There were no changes of MIO-M1 cell viability after 24 h exposure to 20, 30, 40, and 80 μM chrysene (Chry) compared to the dimethyl sulfoxide (DMSO)-equivalent controls. B: After exposure to 30 μM chrysene for 7 days, the MIO-M1 cells showed a decrease of cell viability compared to DMSO-equivalent cells (*p<0.05). The cell viabilities in MIO-M1 cells exposed to 5, 10, and 20 μM chrysene for 7 days were similar to DMSO-equivalent controls and untreated controls. C: There were significant decreases of MIO-M1 cell viabilities after 24 h treatment with 1,000 μM (***p<0.001), 500 μM (***p<0.001), and 300 μM (*p<0.05) chrysene compared to DMSO-equivalent controls. Cultures treated with 100 μM chrysene showed a similar level of cell viability to DMSO-equivalent controls. D: Some cells were pretreatment 6 h with varying concentrations of lipoic acid (LA) and then exposed to 500 μM or 1,000 μM chrysene (Chry +LA) for an additional 24 h. Cell viability levels were not reversed by LA pretreatment in any of the MIO-M1 cells exposed to 1,000 μM chrysene. The loss of cell viability was reversed by pretreatment with 80 μM LA (p<0.001) and 100 μM LA (p<0.001) in the MIO-M1 cells exposed to 500 μM chrysene. Cell viability levels were not decreased in MIO-M1 cultures treated with 1,000 μM DMSO-equivalent, 500 μM DMSO-equivalent, or by exposure to 100 μM LA alone. Assays were performed in triplicate and the experiments repeated three times. Values are mean ± standard error mean (SEM).