Table 1 of
Kumar, Mol Vis 2013; 19:2436-2450. Table 1 of
Kumar, Mol Vis 2013; 19:2436-2450.
Table 1. Oligonucleotides of mutated genes used as primers for PCR amplification.
| Gene Name | Primer Name | Primer Sequence | Anne Temp (°C) | Product Size (bp) |
|---|---|---|---|---|
| CRYBA4 | Ex-2-F | 5′-TAGCCCAGTCACTCCTGGAC-3′ | 57 °C | 213 |
| Ex-2-R | 5′-GCCTTGATTGCACCTCTGTG-3′ | |||
| Ex-3-F | 5′-TTTGCAATCCCTGCTTTACC-3′ | 57 °C | 423 | |
| Ex-3-R | 5′-ATGGCACCCTCCTACTGTTGG-3′ | |||
| Ex-4-F | 5′-AAAAATGTCTCCAGCCATCG-3′ | 57 °C | 314 | |
| Ex-4-R | 5′-AGCTTGAAGTGGCGACATGAG-3′ | |||
| Ex-5-F | 5′-AAATGGCAAGGTTTCTGGTAC-3′ | 57 °C | 297 | |
| Ex-5-R | 5′-GCCTCAGTGTTCTCCTCTGG-3′ | |||
| Ex-6-F | 5′-AGGGAATGGCATGATCAAAG-3′ | 57 °C | 335 | |
| Ex-6-R | 5′-TGCTGGGTTCACACAGGTTAC-3′ | |||
| CRYBB1 | Ex-2-F | 5′-ACAGGATGTGGGGCTATGAG-3′ | 59 °C | 380 |
| Ex-2-R | 5′-GTGCGGAGGAGTAAGAGGTG-3′ | |||
| Ex-3-F | 5′-CATTTCACAAACTGTGGCTCA-3′ | 62 °C | 379 | |
| Ex-3-R | 5′-GGACATAATGTATGTGCCAGGA-3′ | |||
| Ex-4-F | 5′-GTAGGGAGTGGGGGCTTCTA-3′ | 62 °C | 286 | |
| Ex-4-R | 5′-CTCCTTCTTGCCCTTGTCAG-3′ | |||
| Ex-5-F | 5′-GCTCATCTCTCTCGCTCCAC-3′ | 61 °C | 298 | |
| Ex-5-R | 5′-TCTGATTCTGCCTGTGCTTG-3′ | |||
| Ex-6-F | 5′-TCAATGAAGGACAGGCTGGT-3′ | 62 °C | 381 | |
| Ex-6-R | 5′-TCCAGGAGAAATTTTGGCTTT-3′ | |||
| CRYBB2 | Ex-2-F | 5′-CAGAGGGGAGTGGTCTCAAG-3′ | 59 °C | 244 |
| Ex-2-R | 5′-ATGCCAAGCCCATTTTACAG-3′ | |||
| Ex-3-F | 5′-TCAGCATCCTTTGGGTTCTC-3′ | 59 °C | 299 | |
| Ex-3-R | 5′-CAAGGGTAGATTCCCCCACT-3′ | |||
| Ex-4-F | 5′-AACCCTAGGGGTCAACATCA-3′ | 62 °C | 297 | |
| Ex-4-R | 5′-CTCCAAGGTGGCAGAGAGAG-3′ | |||
| Ex-5-F | 5′-GAGTGATGTGTGGGACATGC-3′ | 62 °C | 377 | |
| Ex-5-R | 5′-CAGAGGTCAGCAGAGCACAC-3′ | |||
| Ex-6-F | 5′-GGCTTCACCCTTCCTAGTGG-3′ | 59 °C | 399 | |
| Ex-6-R | 5′-CAAAGACCCACAGCAGACAA-3′ | |||
| GAJ3 | Ex-1A-E | 5′-TGCGGACCCGGCACTCAGC-3′ | 62 °C | 383 |
| Ex-1A-R | 5′-TCCATGCGCACGATGTGCAGTCA-3′ | |||
| Ex-1B-F | 5′-CTGTTCATCTTCCGCATTTTGG-3′ | 62 °C | 603 | |
| Ex-1B-R | 5′-TCTTCTTCCAGCCCAGGTGGTA-3′ | |||
| Ex-1C-F | 5′-AAGCTCAAGCAGGGCGTGAC-3′ | 62 °C | 624 | |
| Ex-1C-R | 5′-CTAGATGGCCAAGTCCTCCGG-3′ | |||
| BFSP1 | Ex-1-F | 5′-GGGCCTCCGGTGTTTATTTA-3′ | 58 °C | 589 |
| Ex-1-R | 5′-ATCGACAGGGGACCGAGAGAC-3′ | |||
| Ex-2-F | 5′-AAAGGAGAGGGCATCGTACC-3′ | 58 °C | 238 | |
| Ex-2-R | 5′-AACCTGCACTTCCACCATTC-3′ | |||
| Ex-3-F | 5′-CAGGTGGTCTGTGTGCACAT-3′ | 58 °C | 249 | |
| Ex-3-R | 5′-TCGGCTTACCTGATCAAACC-3′ | |||
| Ex-4-F | 5′-RGCCATTCCTGTTCTCATCT-3′ | 58 °C | 250 | |
| Ex-4-R | 5′-GCCCTTCCCTGGGAGTCT-3′ | |||
| Ex-5-F | 5′-ACCTTCTCTGCCCTTTTCCT-3′ | 58 °C | 227 | |
| Ex-5-R | 5′-CACCTCCATGAAACATGTGG-3′ | |||
| Ex-6-F | 5′-CCTTTTCCTGGGTGAGGTCTG-3′ | 58 °C | 366 | |
| Ex-6-R | 5′-GGCACACAATAGGCACTCAA-3′ | |||
| Ex-7-F | 5′-CTTGCCCCTGACCTCTGTT-3′ | 58 °C | 199 | |
| Ex-7-R | 5′-AAGAGAGCCGCTTGGTTTTT-3′ | |||
| Ex-8-F | 5′-TTCCAACCAGCGTATTTTCTTT-3′ | 58 °C | 699 | |
| Ex-8-R | 5′-TCAGGGCCTTCCAGCTCT-3′ | |||
| In5-Ex-7-F | 5′-CATCTTCCAGGGTGTCCAG-3′ | 58 °C | 316 | |
| In5-Ex-7-R | 5′-AAGAGAGCCGCTTGGTTTTT-3′ |