Table 1 of
Dong, Mol Vis 2013; 19:2426-2435. Table 1 of
Dong, Mol Vis 2013; 19:2426-2435.
Table 1. Primers used in sequencing of the PRPF31 gene and allele-specific PCR analysis.
| Exon/mutation site or SNP | Primer sequence (5'-3') | TM (°C) |
|---|---|---|
| 2,3 | F: GTCGGGGCAAGTTTTTAGGG | 64 |
| R: GAGATGGGGAGGGGCACAGAGT | ||
| 4 | F: CCGAGAGGGGGTAGGGATTTAGAT | 64 |
| R: AGGCCAGTGGGGAAGGGAGAGG | ||
| 5 | F: TTAGGGCCAACCAGCAGAGTC | 64 |
| R: GAGGGGGTCCGAGATGAGC | ||
| 6,7 | F: GTTCCCGAGCCTCCCCTATCTTCT | 64 |
| R: CGCTCCAGCTCCTCCTCCGACAG | ||
| 8 | F: CCGGCGGCCTGACCAACC | 64 |
| R: GGGAGGGGCCATGACGCAGTG | ||
| 9 | F: GCGCGGTTGCTTTGCTGTTA | 64 |
| R: ACTGCCTCCGCCTTGGTAG | ||
| 10,11 | F: GTGGCGGTGAGGCAGCATTAGGTG | 55 |
| R: CTGGCTGGCTGTGGGGTTGAGGA | ||
| 12,13 | F: GGGCCTGGTCGCTGA | 64 |
| R: GGGGAGGTACCTGGAGTGG | ||
| 14 | F: GGTCACAGTTGGGGCCTTCTCCTC | 64 |
| R: TACTGGGCGGTGATCTCGGTCCTG | ||
| 13/p.G405fs+7X | F: AAGTCGGGCAGTGGC | 60 |
| PRPF31-3A2R | R: AGGTTCACGCCATTCTC | |
| INS-R | R: TAGTCTCATGGACAGGTCTAGCCG | 60 |
| rs4806718 | F: GCCCTCTCATCCTCCCACTT | 58 |
| R: TGCTGAGTGCTGGGACCT |