Figure 3. Deoxyribonucleic acid sequence chromatograms and allele-specific polymerase chain reaction analysis on the mutations detected
in family RP106. A: The heterozygote sequence (sense strand) shows one-base-deletion c.1215delG (p.G405fs+7X) in patient III:1. B: The sequence shows the corresponding wild- type sequence. C: Allele-specific polymerase chain reaction (AS-PCR) analysis shows the amplified products of the mutation allele (382 bp)
cosegregating with patients in this family. The fragments (830 bp), which are the parts of exon 3 of the myocilin (MYOC) gene, were used as the internal control in the AS-PCR analysis.