Figure 8. Integrin-dependent contraction of vitreous explants from chicken embryos. EDTA (EDTA; 5 mM) or heparin (10 μg/ml) was preincubated with human retinal Müller cell line (MIO-M1) cell suspension (A) and bEnd.3 cell suspension (B) for 30 min and then added to the vitreous explants. The vitreous explants were incubated in Dulbecco’s modified Eagle’s medium (DMEM) at 37 °C up to the indicated periods. Thereafter, the wet weight of vitreous explants was measured for quantitative analysis of vitreous contraction. Data are expressed as mean±standard deviation (SD; n=4). ** and ***, significantly different from control (p<0.01 and 0.001, respectively).