Figure 2. Cone degeneration in Rpe65^−/− and Cspg5^−/−/Rpe65^−/− retinas. Central regions of retinas from 8-week-old wild-type (A), Cspg5^−/− (B), Rpe65^−/−, and (C) Cspg5^−/−/Rpe65^−/− (D) mice were analyzed. Cones were labeled with fluorescein-conjugated peanut agglutinin (green), nuclei were stained with 4',6-diamidino-2-phenylindole dihydrochloride (blue), and the images were merged. The cone outer segments were prominently stained in the wild-type and Cspg5^−/− retinas (A, B). Residual staining of cone inner segments was observed in the central retina of the Rpe65^−/− and Cspg5^−/−/Rpe65^−/− mice (C, D). In the peripheral regions of the Cspg5^−/−/Rpe65^−/− retinas, cone outer segment labeling was still observed, with mislocalization to the synaptic endfeet of cone photoreceptors (star; E). Abbreviations: photoreceptor outer segments (os); photoreceptor inner segments (is); outer nuclear layer (onl); outer plexiform layer (opl); inner nuclear layer (inl); inner plexiform layer (ipl); ganglion cell layer (gcl). Scale bar equals 50 μm.