Figure 5. Effect of YC-1 on HIF-1α protein expression under chemical hypoxia. Total proteins were extracted from 661W cells incubated with 0.066% DMSO, 20 µM YC-1, 200 µM CoCl₂, or both YC-1 and CoCl₂ for 2 h (A) and 24 h (B). Expressions of HIF-1α and β-actin (which served as the internal control) were determined with immunoblotting.