Figure 2. LIVE/DEAD cell viability assay of YC-1-treated cells under chemical hypoxia. A: After treatment with 200 μM CoCl₂ in the absence or presence of 20 μM YC-1 for 24 h, 661W cells were stained with calcein AM (green for live cells), EthD-1 (red for dead cells), and Hoechst 33,342 (blue for nuclei). Representative fluorescence staining shows the cell density and composition. B: 661W cells were exposed to 0.066% DMSO, 20 μM YC-1, 200 μM CoCl₂, or both YC-1 and CoCl₂ for 24 h. After fluorescence staining, cells was measured and analyzed with flow cytometry. C: Quantitative data of flow cytometric analysis are from three independent experiments (n=3). * Indicates p<0.05 compared to the control group or DMSO vehicle group; # indicates p<0.05 compared to the chemically induced hypoxic group.