Figure 5. The effects of U-0126 and hydrogen peroxide on ferritin levels in lens epithelial cells and retinal pigment epithelial cells. (A) Lens epithelial cells (LECs) or (B) retinal pigment epithelial (RPE) cells were treated with either 10 μM U-0126 or 250 μM hydrogen peroxide (H₂O₂) or both for 20 h. Cells treated with both were pretreated for 10 min with U-0126 before H₂O₂ was added. Ferritin content of the cells was measured with enzyme-linked immunosorbent assay (ELISA). Each histogram bar represents the mean±SEM of at least six samples. A: *p=0, significantly different from control and U-0126, **p<0.05, significantly different from all other groups. B: *p<0.05, significantly different from control and H₂O₂. **p<0.05, significantly different from all other groups. Statistical tests for all data included ANOVA and Tukey’s test.