Figure 2. U-0126 blocks hydrogen peroxide–induced activation of extracellular signal-related kinase 1/2. Western blot of extracellular signal-related kinase 1/2 (ERK1/2) obtained from retinal pigment epithelial (RPE) cells was probed with antibody specific for ERK1/2 (42 and 44 kDa). Phospho-ERK1/2 expression (upper panel) and ERK1/2 (lower panel) in (A) lens epithelial cells (LECs) and (B) RPE cells treated with 250 μM H₂O₂ in the presence or absence of U-0126 for 24 h.