Figure 5. Collagen fibril diameter distribution: glaucoma compared to control. A histogram of collagen fibril diameter density (fibers/um²) is plotted for the control mouse sclera (green line, n=20 mice; 5 CD1, 5 B6, 5 Aca23-WT, and 5 Aca23) and glaucomatous sclera (blue line, n=19 mice; 5 CD1, 5 B6, 5 Aca23-WT and 4 Aca23; indicating density on the right y-axis). The ratio of the glaucoma density to control density at each diameter is represented as a linear regression (red line: r²=0.62, indicates values of ratio glaucoma to control, left y-axis). The glaucoma group has a higher proportion of smaller collagen fibrils and a lower proportion of larger fibrils with the crossover from more, smaller to fewer, larger fibrils occurring near the mean fibril diameter for controls (vertical dotted line).