Figure 5. Immunohistochemistry for X-linked inhibitor of apoptosis, Thy 1, glial fibrillary acidic protein, and 4',6-diamidino-2-phenylindole in retinal cryosections of young and old eyes at 8 days after induction of glaucoma. The merged image shows colocalization of X-linked inhibitor of apoptosis (XIAP) with Thy 1 (yellow), suggesting that the source for changes in XIAP expression is in the retinal ganglion cell (RGC) layer. A: In 3-month-old eyes, XIAP levels were increased as compared to fellow eyes. B: In old glaucomatous 13-month-old eyes, XIAP staining decreased in the RGC layer as compared to fellow eyes. Magnification 40X, scale bars: all panels 20 μm.