Figure 2. Validation of selected microarray data by quantitative reverse transcription polymerase chain reaction. Relative expression
levels of selected microRNAs (miRNAs) were determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR).
The results showed that the expression of mmu-miR-31, mmu-miR-695, mmu-miR-183, mmu-miR-182, mmu-miR-194, and mmu-miR-190
markedly downregulated in the corneal endothelium of old mice compared to young mice. Meanwhile, the expression of mmu-miR-34c
and mmu-miR-124 markedly upregulated in the corneal endothelium of old mice compared to young mice. Values are mean±standard
deviation (SD) and expressed relative to internal control (U6; n=3 for each group, * p<0.05, Student t test).