Figure 5. Increased immunoglobulin G levels in the aqueous humor of optic nerve homogenate antigen animals. The aqueous humor samples from control (CON) and optic nerve homogenate antigen (ONA) animals were tested for immunoglobulin (IgG) levels 10 weeks after immunization via enzyme-linked immunosorbent assay (ELISA; n=6/group). In the samples of ONA animals, a significantly higher amount of IgG could be detected compared to CON samples (ONA: 34.3±14.4 ng/ml; CON: 13.0±4.1 ng/ml; p=0.006).