Figure 5. Activation of phospho-ERK1/2 and phospho-c-Jun protein in human scleral fibroblasts treated with 1 µmol/l all-trans retinoic acid is inhibited by pre-treatment with 1 µmol/l LE135. As the western blot of panel A shows, incubation with ATRA did not change the expression of phospho-ERK1/2 protein in HSFs pretreated with LE135. The bar graph of panel B shows that the expression of phospho-ERK1/2 protein relative to GAPDH density in cells incubated with ATRA is unchanged after pretreatment with LE135 (n=3). As shown in the western blot of panel C, the expression of phospho-c-Jun protein incubated with ATRA is unchanged in HSFs pretreated with LE135. The bar graph of panel D shows that the expression of phospho-c-Jun protein relative to GAPDH density in cells incubated with ATRA is unchanged after pretreatment with LE135 (n=3).