Figure 1. Primary tissue culture of human scleral fibroblasts (HSFs) and the morphology changes in human scleral fibroblasts treated with all-trans retinoic acid for 24 h. A: HSFs migrated from the sclera tissue after 2 weeks of culture. B: HSFs populated the surroundings and arranged in a radiating pattern for another 1 to 2 weeks. C: Normal group without all-trans retinoic acid (ATRA) cultured for 1 month. D: After being treated with 1 μmol/l ATRA for 24 h, the HSFs shrank, the apparent number of HSFs decreased, and the intercellular space widened compared with the normal group. The original magnification was 100X.