Figure 4 of Nakajima, Mol Vis 2013; 19:174-183.

Figure 4. Pre-treatment with inhibitors of pathways, that are downstream of the action by pituitary adenylate cyclase-activating peptide (PACAP), attenuated PACAP induced neurite outgrowth. A: The graph shows percentage of cells with elongated neurites in trigeminal nerve (TgN) cells stimulated with PACAP alone or with downstream pathway inhibitors U73122 (phospholipase C [PLC] inhibitor) or ddA (adenylate cyclase inhibitor). Data are means±SEM (n=3). *p<0.05 is relative to vehicle (Dunnett’s t test). B: Total mRNA extracted from TgN cells treated with PACAP-27 (black columns) showed higher follistatin expression over vehicle (Nor, gray columns). Initial sample (Ini) was collected immediately before addition of PACAP. Data are means±SD (n=3). *p<0.05 is relative to vehicle (Student t test).