Figure 1. No neovessel formation was observed in low-density lipoprotein receptor–deficient apolipoprotein B-100-only mice overexpressing
insulin-like growth factor II (IGF-II/LDLR–/–ApoB100/100). Retinal whole mounts reveal a normal retinal vasculature in both 15-month-old IGF-II/LDLR–/– ApoB100/100 mice and LDLR–/–ApoB100/100 mice (A and B) fed with a normal diet. Lectin staining of capillaries (C, left panel), Chondroitin Sulfate Proteoglycan (NG2) staining for pericytes (C, middle panel), and a merged image (C, right panel) of control LDLR–/–ApoB100/100 (C, upper panel) and IGF-II/LDLR–/–ApoB100/100 mice (C, lower panel) reveal a normal retinal vasculature in both 15-month-old IGF-II/LDLR–/–ApoB100/100 mice and LDLR–/–ApoB100/100 mice (A and B) fed with a normal diet. The scale bar is 50 µm in A and B, and 25 µm in C.