Table 1 of
Millá, Mol Vis 2013; 19:1707-1722. Table 1 of
Millá, Mol Vis 2013; 19:1707-1722.
Table 1. Primers and PCR conditions used for the amplification of coding exons of MYOC and CYP1B1.
| Fragment | Primer | Sequence (5’-3’) | Annealing temperature | Amplicon size (bp) |
|---|---|---|---|---|
| MYOC-1 | MYOC-1F | CTCTGGAGCTCGGGCATG | 56.9 °C | 753 |
| MYOC-1R | TCACTACGAGCCATATCACC | |||
| MYOC-2 | MYOC-2F | CATAGTCAATCCTTGGGC | 54.5 °C | 380 |
| MYOC-2R | CTGCAGACCTGCTCTGACAA | |||
| MYOC-3A MYOC-3B | MYOC-3AF | GATTTGTCTCCAGGGCTGTC | 58.4 °C | 724 |
| MYOC-3AR | CTGCTGAGGTGTAGCTGCTG | |||
| MYOC-3BF | TCTGTGGCACCTTGTACACC | 56 °C | 994 | |
| MYOC-3BR | ACATCTCCCAACCTGAAGGAA | |||
| CYP1B1–2 | CYP1B1–3F | GGCGCCCGCTCCTGTCTCTG | 61.4 °C | 1216 |
| CYP1B1–3R | ACCTGGAGCGAAACCCCAAA | |||
| CYP1B1–3 | CYP1B1–4F | AATGTGCTTTCTAGATGAAATAAGAA | 54.6 °C | 751 |
| CYP1B1–4R | CAGCACAAAAGAGGAACTGGA |