Figure 7. Effect of resveratrol and sirtinol on the survival of RGC-5 cells in serum-free media were shown. The control group had cells cultured in 10% fetal bovine serum; the label S(-) indicated a group with cells cultured in serum-free media. The group with resveratrol addition showed more cell viability than the group with PBS addition in cells cultured in serum-free media. Coaddition of resveratrol and sirtinol resulted in lower cell viabilities than the group with resveratrol only at 24 h and 48 h after addition. (G; n ≥ 3 for each group, *p < 0.05, one-way analysis of variance [ANOVA], post hoc Tukey test, error bars represent standard deviation) Immunocytochemistry showed the expression of Brn-3 (A), neurofilament (B) and Thy-1 (C) in RGC-5 cells. The negative controls of Brn-3 (D), neurofilament (E) and Thy-1 (F) were shown, which were stained with only secondary antibody.