Appendix 2 of Li, Mol Vis 2013; 19:1656-1666.


Appendix 2. LY294002 inhibited the AST-induced cytoprotective effect on cell apoptosis against H2O2 in ARPE-19 cells.

To access the data, click or select the words “Appendix 2.” (A) The ARPE-19 cells were incubated with different concentrations of AST (10 µM and 20 µM) for 24 h and then treated with or without 10 µM LY294002 for 30 min before incubation with or without 200 µM H2O2 for 24 h. Flow cytometry recording shows the apoptosis rate of ARPE-19 cells. (B) Summarized data showing the rate of apoptotic cells detected by flow cytometry. Data are shown as mean ± SD (n=6); *P <0.05 vs control; # P <0.05 vs H2O2-induced cells without treatment with AST; §P<0.05 vs H2O2 + 20 µM AST.