Figure 5. Super-resolution confocal microscopy of ankyrin repeat and suppressor of cytokine signaling box containing protein-10-stained structures colocalized with biomarkers of the ubiquitin-proteasome system and autophagy-lysosomal pathways. Human trabecular meshwork (HTM) cells were immunostained with the rabbit polyclonal ankyrin repeat and suppressor of cytokine signaling (SOCS) box containing protein-10 (ASB10) antibody (red; A, B, D–F), the goat polyclonal ASB10 antibody (red; C), total ubiquitin (green; B), K63-linked ubiquitin (green; C), histone deacetylase 6 (HDAC6, green; D), light chain 3 (LC3, green; E), and Rab7 (green; F). Scale bars=5 µm.