Figure 4. Ankyrin repeat and suppressor of cytokine signaling box containing protein-10 colocalizes with autophagy-lysosomal pathway biomarkers in human trabecular meshwork cells. A: A schematic shows the sequential steps and biomarkers of the autophagy-lysosomal (AL) pathway. B–G: HTM cells were immunostained with the rabbit ankyrin repeat and suppressor of cytokine signaling (SOCS) box containing protein-10 (ASB10) polyclonal antibody (red; B–G) and histone deacetylase 6 (HDAC6, green; B; aggresomes), heat shock protein 70 (HSP70, green; C; aggresomes), p62 (green; D, autophagosomes), light chain 3 (LC3, green; E, autophagosomes), Rab7 (green; F, late endosomes/amphisomes) or lysosomal-associated membrane protein 1 (LAMP1, green; F, lysosomes). Nuclei were stained with 4',6-diamidino-2-phenylindole dihydrochloride (DAPI). Scale bars=20 µm.