Figure 1 of Contín, Mol Vis 2013; 19:1614-1625.


Figure 1. Effect of constant low light exposure on the number of outer nuclear layer nuclei. A: Retinas reared in a 12:12 h light (200 lx) dark cycle (LD Cycle). Photoreceptors in ONL were morphologically normal. B: Retinas from rats exposed to ten days of constant 200 lx light (LL). These ONL became thinner than the ONL in A. ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer. Scale bar indicates 30 µm. C: Numerical quantification of ONL nuclei. Rat retina images corresponding to the LD cycle, non-exposure to light (DD) or exposure to 200 lx light (LL) for 1,2,4,7, 9 and 10 days were analyzed to quantify photoreceptor survival. The number of nuclei in the different areas (left, middle left, middle right and right) of the ONL was counted using the Automatic Nuclei Counter plug-in (v1.45) for ImageJ software with 40×magnification. Data are mean ± SD (n=2 animals/group) from five independent experiments, p=0.025, by a one-way ANOVA; *:p<0.05 by Duncan’s post- hoc test (LL 7, 9 and 10 D versus control in the LD cycle).