Molecular Vision: Kabir, Mol Vis 2013; 19:1554-1564. Table 1

Table 1. Primer sequences used for bi-directionally sequencing of RPE65. The amplification conditions are described in the methods and materials.

Exon	Forward Primer	Reverse Primer	Annealing Temperatures (°C)
1	GAGAGCTGAAAGCAACTTCTG	ATAGCACATTTATCATGAATCCATG	54
2	CTATCTTGCGGACTTTGAGC	GCCAGAGAAGAGAGACTGAC	60
3	GGCAGGGATAAGAAGCAATG	CTGAGTTCAGAGGTGAAAAC	63
4–5	CTGTACGGATTGCTCCTGTC	TTAGAATCATACATTCGCAGCATG	57
6	TATAATGTATCTTCCTTCTCTCAAC	CTCACAATACAGTAACTTTCTCAC	57
7–8	AAATAAGAGGCTGTTCCAAAGC	TTAAACACATCTTCTTCAGAATCAC	54
9	GTACACTTTTTTCCTTTTTAAATGCATC	GTTTTAGATGTGATTCAGATTGAGTG	57
10	TTGTCATTGCCTGTGCTCATG	TGAGAGAGATGAAACATTCTGG	57
11	TCTGCATTTCTGGCTGTTTG	AAGTGATTCAAGCCAAGTCCA	55
12–13	CACACGGGAGTGAACAAATG	CAACCTTACTCCTTTCCTAACGA	55
14	AGTCAGAAAAAGAAGTCAGGTC	ATTGCTTGCTCAACTCAGTGC	60