Figure 4. Sequence chromatograms of RPE65 variations identified in families PKRP020 and PKRP0160. A: Unaffected individual 10 of family PKRP020 homozygous for the wild-type. B: Unaffected individual 12 of family PKRP020 heterozygous carrier and, C: affected individual 13 of family PKRP020 homozygous for the G to A transition in intron 2; c.95–1G>A. D: Unaffected individual 7 of family PKRP160 homozygous wild-type. E: Unaffected individual 6 of family PKRP160 heterozygous carrier and, F: affected individual 9 of family PKRP160 homozygous for a T to C transition in exon 3: c.179T>C.