Figure 5. Macrophage infiltration analyzed in conjunctival tissue. A: Cluster of differentiation 68 (CD68) positive cells in slides of vehicle (upper; magnification: left 5X, right: 40X) and
azithromycin (lower; magnification: left 5X, right: 40X). The azithromycin-treated group showed a decrease in the number of
macrophages compared with the vehicle-treated animals. CD68 (macrophages): green; 4',6-diamidino-2-phenylindole (nuclei):
blue. B: Results obtained for counting of CD68 positive cells in immunofluorescence slides. Data are represented as mean±standard
error of mean (SEM). Four eyes per group were included. All data were analyzed with SPSS 15.0 software. (*p<0.05 versus vehicle).