Figure 5. Effect of glucocorticoid receptor silencing on mapracorat inhibition of interleukin-4/interleukin-13 plus tumor necrosis factor-α-induced
cytokines and intercellular adhesion molecule 1 in human conjunctival epithelial cells and human conjunctival fibroblasts.
After glucocorticoid receptor (GR) knockdown, cells were treated with interleukin-4 (IL-4) plus tumor necrosis factor-α (TNF-α)
(human conjunctival epithelial cells [HConEpiC]) or IL-13 plus TNF-α (human conjunctival fibroblasts [HConF]) in the presence
of mapracorat or dexamethasone. A: GR protein expression was determined with western blotting. B: Regulated on activation, normal T cell expressed and secreted (RANTES) levels and intercellular adhesion molecule 1 (ICAM-1)
expression in HConEpiC were determined by Luminex. C: RANTES and eotaxin-1 levels in HConF were determined by Luminex. Left panels show data for control small interfering RNA
(siRNA); right panels show data for GR siRNA. Data are presented as mean ± standard error of the mean (SEM), n=4. * versus
IL-4/IL-13 plus TNF-α; p<0.05.