Figure 6. Lithium chloride promotes cell apoptosis in vitro. A and B: Apoptosis in lithium chloride– (LiCl-) versus sodium chloride– (NaCl-) treated murine macrophage-like RAW264.7 cells (A) and mouse bone marrow–derived neutrophils (B) before Pseudomonas aeruginosa (PA) infection (PA-) and after PA infection (PA+) was analyzed with flow cytometry with annexin V and propidium iodide (PI)
staining. Western blot was used to detect the protein levels of cleaved caspase-3, cleaved poly(ADP-ribose) polymerase (PARP),
P-GSK3β, and β-catenin in murine macrophage-like RAW264.7 cells (C) and bone marrow–derived neutrophils (D) at 12 and 24 h after PA infection. Data represent three individual experiments.