**Figure 4. **Apoptosis in the infected corneas assessed with flow cytometry. **A**: The apoptotic ratios in lithium chloride– (LiCl-) versus sodium chloride (NaCl)–treated corneas at 1 and 5 days p.i. were
detected with propidium iodide (PI) staining associated with flow cytometry, as calculated by the average percentage of PI-positive
cells in the infected cornea (**D**). The respective apoptotic ratios of macrophages (**B**) and neutrophils (**C**) in LiCl- versus NaCl-treated corneas at 1 and 5 days p.i. were analyzed with flow cytometry associated with triple staining
for PI, F4/80, and Gr-1, as calculated by the average percentage of PI-positive cells in either F4/80-positive macrophages
(**E**) or Gr-1-positive neutrophils (**F**). **G**: The percentages of the macrophages and neutrophils in the corneal infiltrating cells were determined with F4/80 and Gr-1
staining associated with flow cytometry. Data are the mean±standard error of the mean (SEM) and represent three individual
experiments (n=5). ***, p<0.001.