Figure 2. Lithium chloride regulates inflammatory cytokine production in vivo. Relative messenger ribonucleic acid (mRNA) expression
levels (fold changes) of interleukin-10 (IL-10) (A), IL-6 (B), IL-1β (C), and tumor necrosis factor-alpha (TNF-α) (D) in lithium chloride– (LiCl-) versus sodium chloride (NaCl)–treated corneas at 1 and 5 days p.i. were tested using real-time
polymerase chain reaction (PCR). Protein levels of IL-10 (E) and TNF-α (F) in LiCl- versus NaCl-treated corneas at 1 and 5 days p.i. were tested using enzyme-linked immunosorbent assay (ELISA). Data
are the mean±standard error of the mean (SEM) and represent three individual experiments with five animals/group/time. *,
p<0.05; **, p<0.01; ***, p<0.001.