Figure 4. Lack of non-homologous recombination. A: An illustration of the 16 kb mouse mitochondrial genome shows potential insertion of human ND4 (blue arrow) between the tRNAs and rRNAs, cytochrome oxidase subunit 1 (B), or ND5 (C). D: Next-generation sequencing reads of the entire mouse mitochondrial genome of AAV-ND4-, MTS-AAV-ND4-, or AAV-GFP-injected eyes were identical and showed no insertions of human ND4 bases into non-homologous sites of the host genome.