Figure 3. Small-scale recombination. A: An illustration shows the nucleotides TTA at positions 144–146 of mouse ND4. B: An illustration shows the CCT codon of human ND4 occupying positions 144–146 of murine ND4 in next-generation sequencing reads of mouse ND4 from eyes injected with MTS AAV-ND4. C: An alignment of ND4 protein amino acids shows that the CCT substitution for TTA results in a transition of tyrosine (Y) for leucine (L) at amino acid 49 of mouse ND4. D: An illustration shows nucleotides AAAC at positions 1360–1363 of mouse ND4. E: An illustration shows human nucleotides CCGA at positions 1360–1363 of mouse ND4 in MTS AAV-ND4-injected eyes. F: An alignment of ND4 amino acids shows that the human CCGA substitution for mouse AAAC would result in a transition of threonine (T) for lysine (K) at amino acid 452, as well as a transition of glutamic acid (E) for leucine (L) at amino acid 453. G: An illustration shows the nucleotides ATA at positions 1375–1377 of mouse ND4. (H) An illustration shows the nucleotides ATA of human ND4 at positions 1375–1377 of mouse ND4 next-generation sequencing reads in MTS AAV-ND4-injected eyes. I: Substitution of human CCT for murine ATA would result in a transition of proline (P) for isoleucine (I) at the last amino acid 459 of mouse ND4.