Figure 2. Annexin V-fluorescein isothicyanate/propidium iodide analysis of apoptosis in HLE-B3 cells treated with SB216763. Cells were treated with annexin V-fluorescein isothicyanate/propidium iodide either in the presence or absence of SB216763. Top row: DMSO control cells without annexin V-fluorescein isothicyanate/propidium iodide is shown. Second row: (left panel) Image of SB216763 treated cells additionally stained with annexin V-fluorescein isothicyanate/propidium iodide; (right panel) higher magnification. Third row: (left) Image of cells stained with annexin V-fluorescein isothicyanate/propidium iodide only; (right) higher magnification. Bottom row: (left panel) Image of SB216763 treated cells; (right panel) higher magnification. Black arrows indicate false fluorescence from undissolved particulate SB216763.